The influence of p53 mutation status on the anti-cancer effect of cisplatin in oral squamous cell carcinoma cell lines
조득원, 김영균, 윤필영,
소속 상세정보
조득원 ( Jo Deuk-Won ) - Seoul National University Bundang Hospital Section of Dentistry Department of Prosthodontics
김영균 ( Kim Young-Kyun ) - Seoul National University Bundang Hospital Section of Dentistry Department of Oral and Maxillofacial Surgery
윤필영 ( Yun Pil-Young ) - Seoul National University Bundang Hospital Section of Dentistry Department of Oral and Maxillofacial Surgery
KMID : 0355620160420060337
Abstract
Objectives: The purpose of this study was to evaluate the anti-cancer activity of cisplatin by studying its effects on cell viability and identifying the mechanisms underlying the induction of cell cycle arrest and apoptosis on oral squamous cell carcinoma (OSCC) cell lines with varying p53 mutation status.
Materials and Methods: Three OSCC cell lines, YD-8 (p53 point mutation), YD-9 (p53 wild type), and YD-38 (p53 deletion) were used. To determine the cytotoxic effect of cisplatin, MTS assay was performed. The cell cycle alteration and apoptosis were analyzed using flow cytometry. Western blot analysis was used to detect the expression of cell cycle alteration- or apoptosis-related proteins as well as p53.
Results: Cisplatin showed a time- and dose-dependent anti-proliferative effect in all cell lines. Cisplatin induced G2/M cell accumulation in the three cell lines after treatment with 0.5 and 1.0 μg/mL of cisplatin for 48 hours. The proportion of annexin V-FITC-stained cells increased following treatment with cisplatin. The apoptotic proportion was lower in the YD-38 cell line than in the YD-9 or YD-8 cell lines. Also, immunoblotting analysis indicated that p53 and p21 were detected only in YD-8 and YD-9 cell lines after cisplatin treatment.
Conclusion: In this study, cisplatin showed anti-cancer effects via G2/M phase arrest and apoptosis, with some difference among OSCC cell lines. The mutation status of p53 might have influenced the difference observed among cell lines. Further studies on p53 mutation status are needed to understand the biological behavior and characteristics of OSCCs and to establish appropriate treatment.
키워드
Cisplatin; p53; Oral squamous cell carcinoma; Apoptosis
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